Journal: Oncoimmunology

Article Title: Increased sMICA and TGFβ 1 levels in HNSCC patients impair NKG2D-dependent functionality of activated NK cells

doi: 10.1080/2162402X.2015.1055993

Figure Lengend Snippet: Cytokine levels and NK cell cytotoxicity of HNSCC patients at diagnosis and upon follow-up. In recurrent head-and-neck squamous cell carcinoma (HNSCC) patients (n=7) the patient plasma sMICA/TGFβ1 levels were determined by ELISA and NKG2D expression on patient natural killer NK cells were measured directly via leukocyte quantification at initial diagnosis (initial) and after relapse (relapse). Additionally, NK cell stability upon co-culturing with SCC-4 cells (4 h, 37°C, at an effector-to-target [E:T] cell ratio of 10:1) and resultant cytotoxicity of the same patient blood NK cells were determined by cytofluorimetric analysis.. Statistical analyses was performed by Mann-Whitney nonparametric U-test and Student's t-test; P ≥ 0.05 was defined as statistically non-significant (n.s.).

Article Snippet: 20 The MTPL ELISA for the quantitative measurement of TGFβ 1 in human blood plasma was purchased from Milenia Biotec (Version 3.0, Germany).

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, MANN-WHITNEY